enzymes barcode adapter ligation Search Results


98
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New England Biolabs adapter ligation

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Image Search Results


Journal: eLife

Article Title: Genetic interactions of G-quadruplexes in humans

doi: 10.7554/eLife.46793

Figure Lengend Snippet:

Article Snippet: To monitor protein levels, cells transfected simultaneously with the same siRNA-reagent mixture were harvested 48 hr and 144 hr after transfection, by cell scraping and lysed on ice (30 min) with RIPA lysis buffer with protease inhibitor +EDTA (Thermo Fisher Scientific, cat # 8990).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Plasmid Preparation, shRNA, Genome Wide, Sequencing, Adapter Ligation, Biomarker Discovery, Transfection, Western Blot, Marker, Library Quantification, Purification, Lysis, Protease Inhibitor, Software